Document Details

Document Type : Thesis 
Document Title :
“RNA EDITING OCCURS IN PLASTOME OF CATHARANTHUS ROSEUS”
تحورات حمض الريبوز النووي لجينوم البلاستيدات الخضراء في نبات الونكا Catharanthus roseus
 
Subject : faculty of science 
Document Language : Arabic 
Abstract : RNA editing is a vital process of post-transcriptional modification that occurs widely occurs in plant organelles. Although, RNA editing methodical studies have been conducted in various plants, very few information is available on Catharanthus roseus. In the present study, editing events in photosynthesis complexes were examined and compared among different tissues, flower, leaves, stems, roots, hairy roots, and seedling of C. roseus. Transcriptome data of C. roseus plastome genes and RNA-Seq were downloaded from the National Center for Biotechnology Information (NCBI) and reference was assembled using bioinformatic tool. In total 80 C-to-U editing sites were identified including 35 non-synonymous and 45 synonymous edited sites. Leaves and stems tissues have the most frequent editing sites with 65 and 66 edits respectively, followed by seedlings with 64 edits of the total edited sites. By contrast, some of the editing sites were significantly reduced in the remaining tissues; flowers (59), roots (59) and hairy roots (55). A comparison of C. roseus editing sites of 18 plant species led to discovery of 50 novel editing sites in 11 genes. Furthermore, affected amino acids were analyzed and compared among the six different tissues. These analyses exhibited that RNA editing increased the hydrophobicity of the total converted amino acids for approximately 82%. The findings show that RNA editing in plastid plastome of C. roseus is tissue dependent and differentially regulated in different types of tissues. 
Supervisor : Dr. Ahmed Mohamed Ramadan Emara 
Thesis Type : Doctorate Thesis 
Publishing Year : 1441 AH
2020 AD 
Co-Supervisor : Dr.Thana Khan 
Added Date : Monday, June 22, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
وسيمة بريكان الشمريAlshammari, Wasimah BuraykanResearcherDoctorate 

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