Document Details

Document Type : Thesis 
Document Title :
A NOVEL APPROACH TO GENERATE VACCINES FOR AVIAN INFLUENZA H5N8 AND MEDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS (MERS-COV) USING SECRETED BACTERIAL OUTER MEMBRANE VESICLES.
توجه حديث لإنتاج لقاحات ضد فيروس انفلونزا الطيور ومتلازمة الشرق الأوسط التنفسية باستخدام حويصلات الغشاء الخارجي للبكتريا
 
Subject : faculty of science 
Document Language : Arabic 
Abstract : Middle East respiratory coronavirus MERS-CoV is a novel coronavirus emerged in 2012 in Saudi Arabia and caused high infected and mortality rates in humans and spread to 27 other countries. Also, Avian influenza H5N8 was first detected in January,2014 in South Korea and spread to most of the world including Saudi Arabia in 2016. A novel approach for vaccine preparation was introduced her using outer surface membrane vesicles OMVs which gave promising results. To accomplish this work, the hemagglutinin 5 H5 from strain of H5N8 and Spike protein S from MERS-CoV were amplified by Reverse Transcriptase Polymerase Chain Reaction RT-PCR and cloned in gene expression vectors, then, these two constructed were transformed in competent cells separately and propagated in liquid medium. The competent cells were sedimented and the outer membrane vesicles were isolated and concentrated from supernatant by specific membranes. After that, animal immunization carried out by inject with OMVs as a vaccine. Then, efficiency of OMVs vaccine were determined by serological assays and compared with inactivated vaccine as well as negative control group. The results showed that the vaccine with OMVs that consists of S and H5 have adequate protection for animals. These results indicate to The OMVs based vaccine technology is a promising approach for combating to emerging epidemic cases. 
Supervisor : Prof. Mohammed Ishtiaq Qadri 
Thesis Type : Doctorate Thesis 
Publishing Year : 1441 AH
2019 AD 
Co-Supervisor : Prof. Fahad Abdulrhaman Al-Fassi 
Added Date : Sunday, December 8, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ثامر أحمد بوبكBouback, Thamer AhmadResearcherDoctorate 

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