Document Details

Document Type : Thesis 
Document Title :
EFFECT OF WATER POLLUTION ON THE ANTIBIOTIC RESISTANCE OF FISH MICROBIOTA
تأثير تلوث المياه على مقاومة المضادات الحيوية للجراثيم السمكية
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : The gut microbiota is maintaining the symbiotic relationship between the host and the intestinal microbiome including bacteria, archaea, viruses and protozoa. Dietary habits, lifestyle, age, host genotype and exposure to antibiotics may affect the composition of the gut microflora. The current study was targeted to explore the influence of pollution oxidative stress on the incidence of ESBL carrier species in wild fish species from the Red Sea costal area of Jeddah City in Saudi Arabia against multiple drugs, cephalosporins and carbapenems. Antibiotic susceptibility test had been performed using cephalosporins and carbapenems against ESBL and CRE producing bacteria. DNA was extracted form purified colonies and was used for 16S rDNA amplification. The isolates were tested for the common genes responsible for the ESBL+ phenotype. Primers coding for ESBL (blaCTX, blaSHV, blaTEM, and blaOXA) were used to detect ESBL+ phenotype by sequencing the PCR products. Screening results from the polluted site revealed ESBL resistant Klebsiella pneumoniae B8; and CRE resistant Morganella morganii A4. The presence of Morganella morganii in the polluted site had developed the blaCTX-M gene which could be used as a pollution marker. The other isolates Acinetobacter pittii and Providencia rettgeri were found in the CR site. 
Supervisor : Dr. Othman A. Baothman 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2019 AD 
Co-Supervisor : Prof. Youssri Mohamed Soliman 
Added Date : Thursday, November 21, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
يونس عبدالخالق الشمرانيAlshamrani, Yonus AbdulkhaliqResearcherMaster 

Files

File NameTypeDescription
 45174.pdf pdf 

Back To Researches Page