Document Details
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Document Type |
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Thesis |
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Document Title |
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Identification of Regulatory Mechanisms of Early Stages of Flower Developmental in Tomato (Solanum lycopersicum) تحديد الميكانيكيات المنظمة للمراحل المبكرة لنمو الأزهار في الطماطم (Solanum lycopersicum) |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Tomato (Solanum lycopersicum L.) is among the most important vegetable crops worldwide. Functional genomic analysis of early transcript genes in the earlyfruit stages are the key factors for enhancing tomato fruits for increased yield and quality. In the present study, RNA-Seq datasets were generated for the Chico cultivar at six growth stages, 0 days after pollination (DAP), 3, 5, 7, 9 and 12 DAP. Annotated transcripts and KEGG analysis indicated that starch and sucrose pathway experienced the most dynamic change resulting in the synthesis of several soluble sugars, e.g., sucrose, -D-fructose, -D-glucose, trehalose, maltose and 1,4--D-xylan. Enzymes activated in this pathway included levansucrase, phosphotransferse, invertase, pectin methyl esterase (PME2.1), SPH1 synthase and cellulase. RNA-Seq datasets were also utilized to detect TEOSINTE BRANCHED 1, CYCLOIDEA, and PROLIFERATING CELL FACTORS (TCP) transcription factors and their concordantly expressed genes. The data indicated the regulation of 23 TCP-encoding genes during fruit development. Five of these TCPs showed concordant expression with other transcription factors and genes. These TCPs exist in class I (TCP12, TCP15 and TCP18), and class II, e.g., CIN (TCP24) and CYC/TB1 (TCP9). TCP12 and TCP18 participate in fruit development and/or ripening, while TCP15 participates in auxin biosynthesis. TCP24 gene showed no concordant expression with TCP12, TCP15 or TCP18 genes, while showed concordant expression with XpoI and cdc5 genes that participate in initiation of programmed cell death as early as at fruit development stages. The results indicated the possible role of TCP9 in fruit ripening as it might drive expression of MADS-RIN as well as AO3 gene that catalyzes the oxidation of abscisic aldehyde to ABA, processes functioning during fruit development and ripening. TCP15 indicated the concordant expression with auxin response factor 5 (ARF5) functioning in the regulation of tomato fruit set and development. In conclusion, the study scopes the light on the possible mechanisms underlying fruit characteristics that can be used in improving fruit yield and quality. |
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Supervisor |
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Dr. Rania Mackie |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2019 AD |
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Added Date |
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Thursday, September 19, 2019 |
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Researchers
| إلهام معيض الشمراني | Alshamrani, Elham Muedh | Researcher | Master | |
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