Document Details

Document Type : Thesis 
Document Title :
Identification of methicillin-resistant Staphylococcus aureus among medical students in Jeddah using conventional and molecular methods
كشف المكورات العنقودية الذهبية المقاومة للميثيسيلين بين طلاب الطب بجدة باستخدام الطرق التقليدية والجزيئية
 
Subject : Faculty of medicine 
Document Language : Arabic 
Abstract : Infections can be acquired from hospitals (nosocomial) or from the community. Nasal carriage of Staphylococcus aureus (S. aureus) is very common especially among hospital staff such as doctors, nurses, laboratory technologists and also among medical students during their clinical training years. Nasal carriage of S. aureus is present in 25% among general population and can be transmitted by hand-to-nose route. This microorganism can be pathogenic when acquires virulence factors. Among these factors is acquiring mecA gene which is responsible for methicillin-resistance. Public health worldwide is concerned about the emergence of MRSA strains because it was only confined to hospitals but nowadays it is present in the community as well. MRSA has been detected in Saudi Arabia since 1990s. In the present study 200 Samples were collected from sixth year medical students and interns at King Abdulaziz University Hospital then tested for S. aureus carriage by conventional and molecular (PCR) methods. The prevalence of nasal colonization by S. aureus was 25% [of which 18% were colonized by MSSA (methicillin-sensitive S. aureus) and 7% by MRSA]. Both methods can identify S. aureus. PCR is excellent in the detection of mecA gene but not in the assessment of mecA genomic activity (phenotypic resistance to methicillin) which is done by antimicrobial-susceptibility testing of the conventional method. Educational sessions for medical students and hospital staff on patient’s safety and hand washing are recommended. 
Supervisor : Prof. Asif Ahmad Jiman-Fatani 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Added Date : Wednesday, July 31, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
طارق سعد اخميميEkhmimi, Tariq SaadResearcherMaster 

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