Document Details

Document Type : Thesis 
Document Title :
Enhancement of biologically active exopolysaccharides production by Bacillus firmicutes
تعزيز إنتاج السكريات العديدة الخارجية النشطة حيوياً بواسطة باسيلوس فيرميكوتس
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Our worked deal with the production of exopolysaccharide by bacteria from dates seed powder as solo carbon source. A pure bacterial colony was isolated from hot spring and identified by phylogenetic as B. firmicutes. Bacterial strain, B. firmicutes was capable of producing EPS using very cheap carbon ago seeds pits as solo carbon source. Important parameters were studied to enhance exopolysaccharide production. Maximum total sugars (exopolysaccharide) were reached to 0.87 mM at 20 g/L agwa dates seed (ADS). The maximum production was found to be 3.46 mM by addition of peptone as main source of nitrogen with concentration 1.5 g/L. The optimal parameter values were temperature 37°C, pH 6, incubation time 72h and inoculum concentration 1 ml. The crude exopolysaccharide was purified by removing the cells, then the protein, dialysis the exopolysaccharide solution then precipitation by ethanol. The purified exopolysaccharide about (0.5 g) was hydrolyzed and analyzed by HPLC. The components of monosaccharides were glucouronic acid, fructose and glucose with a molar ratio of 2.2: 1.3: 1.0 respectively. Furthermore, it also proved that B. firmicutes exopolysaccharide showed significant activity as antioxidant as detected by DPPH and antiproliferative activity on Ehrlich tumor. 
Supervisor : Prof. Dr. Youssri M. Ahmed Soliman 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Co-Supervisor : Dr. Wesam H Abdulaal 
Added Date : Wednesday, July 24, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
راكان هشام يوسفYousef, Rakan HishamResearcherMaster 

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