Document Details

Document Type : Thesis 
Document Title :
Isolation of a bioactive compound from Serratia marcescens bacteria
عزل مركب نشط حيويا من بكتيريا السريتيا مرسيسنس
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Gram negative, short rods, mostly single forms Serratia marcescens was isolated from Red Palm Weevil (Rhynchophorus ferrugineus) infected by the entomopathogenic fungus Beauveria bassiana. Serratia marcescens was found to produce the bio-active prodigiosin. This work seeks to optimize prodigiosin production Serratia marcescens. Peanut powder and peptone were identified as the optimized carbon and nitrogen sources for the production of prodigiosin, yielding a prodigiosin concentration in the supernatant (1581mM) and 586 mM in cells debris. To enhance prodigiosin production even further experimental design methodology was utilized to optimize the environmental condition as temperature which was observed at 28°C. Optimum pH was 7, agitation allows the oxygen to dissolve in the medium it was observed that 50 ml medium is optimum volume for prodigiosin production. It was observed that the optimize of incubation time was 72 h maximum level of prodigiosin. Crude extract of pigment was further purified by silica gel column chromatography. One spot was detected by thin layer chromatography was identified as prodigiosin. The acid and alkali prodigiosin were exposed ultraviolet-visible scan. The acid prodigiosin gave maximum absorption at 537 nm and the maximum absorption in alkaline was 468 nm. Mass spectrometry revealed a molecular weight of prodigiosin is 324 Da. By detection the antioxidant activity, it was found A reduction of 50% of the absorbance (IC50) was achieved at 0.19 μg /ml concentration of prodigiosin pigment extract. Purified pigment had antimicrobial properties against Gram-positive bacteria E.faecalisis, S.aureus, B.subtilis, Gram-negative bacteria E.coli, K.pneumoniae, P.mirabilis, P.aeruginosa and yeast S.cerevisiae, Candida albicans. 
Supervisor : Prof. Dr. Youssri M. Ahmed Soliman 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Co-Supervisor : Dr. Othman A. Baothman 
Added Date : Wednesday, July 24, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عيسى عودة البلويAlblwi, Eisa OdahResearcherMaster 

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