Document Details
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Document Type |
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Thesis |
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Document Title |
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MOLECULAR AND IMMUNOLOGICAL EFFECT OF PROBIOTIC BACTERIA IN MICE COLON CANCER INDUCED BY AZOXYMETHANE. التأثير الجزيئي و المناعي لبكتريا البروبيوتك على سرطان قولون الفئران |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Colorectal cancer is the commonest cancer in human worldwide where its incidence may correlate with diet, lifestyle, and hereditary factors. There has been an upsurge in research into probiotics and a suggestion that they could influence disease severity and prevent cancer. To exhibit such effects on the histological ,molecular and immunological levels in mice intestine and serum, sixty female mice were utilized and divided equally into 6 groups included; a untreated group (C); a group of mice was injected with Azoxymethane alone to induce colon cancer (CC); two groups of mice orally administrated with either Lactobacillus (Lac) or Streptococcus probiotic strains (Strep); two groups of mice sustained exposure to either Lactobacillus (Lac CC) or Streptococcus (Strep CC) bacterial strains pre and post injection with Azoxymethane. TLR2, IFN-γ, IL-10 and IL-13 genes expression were verified a significant upregulation in Lac mice intestine compared to Strep group. Both Lac and Strep mice groups showed a significant upregulation in mucosal IL-4-RNA. Although TNFα in mice sera increased significantly in Lac mice serum, IL-2 elevated significantly in Strep mice serum group. Interestingly, Lac CC mice group demonstrated an early stimulation in TLR2, IFN-γ, IL 4, IL 10, and IL 13 mRNA expression in mice intestine biopsy compared to Strep CC, CC, and C mice groups. Notably, P53 gene expression confirmed a significant downregulation in the early stages of adenocarcinoma either in CC or Strep CC mice groups compared to Lac CC mice while K-Ras gene expression upregulated significantly in all treated mice groups. All immunological and molecular results have been confirmed by histological findings where, Strep CC and CC mice groups showed a development of colon adenocarcinoma which characterized by the loss of goblet cells and elongated nuclei with occasional cystic Lumina at the surface epithelium, while Lac CC group showed no alterations in the histological structure, which has been seemed similar to the untreated control group. Molecular, immunological and histological parameters established that Lactobacillus probiotic strain inhibits tumor growth and its anti-cancer activity occurs, at least in part, while Streptococcus probiotic strain cannot be used as a prophylactic treatment to prevent or inhibit mice colon cancer development. |
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Supervisor |
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Dr. Sahar Ragab El Hadad |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2018 AD |
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Co-Supervisor |
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Dr. Alia M. Aldahlawi |
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Added Date |
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Sunday, November 11, 2018 |
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Researchers
| مها ذياب السلمي | Al-Solami, Maha Diab | Researcher | Master | |
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