Document Details
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Document Type |
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Thesis |
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Document Title |
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EXPRESSION OF ONCOGENIC LONG NON-CODING RNAS IN COLORECTAL CANCER PATIENTS التعبير الجيني للحمض النووي الريبوزي الطويل المسرطن والغير مشفر في مرضى سرطان القولون والمستقيم |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Colorectal cancer (CRC) is the third most common cancer and the fourth leading cause of cancer death in the world. In KSA, CRC represents the first and the third most common cancer type in males and females, respectively. Despite advances in current detection methods, more than half of patients die at advance stage of disease and in many cases diagnosis of CRC occurs at late stage when the cancer spreads in other organs of the body and metastasis appears. Therefore, there is an urgent need to understand molecular mechanism of CRC and develop effective biomarkers that have critical role in detection and treatment of CRC. Sensitivity of long non-coding RNAs (lncRNAs) in diagnosis is more specific than DNA, protein coding RNA and protein biomarkers. This study aimed to measure the expression of selected oncogenic lncRNAs (PANDAR, MALAT1, PCAT6, CCAT1, UCA1, MEG3, CCAT2 and BCAR4) in blood samples of healthy individuals and CRC patients and to correlate these tested lncRNAs, which showed significant expression if any, with the tumorigenesis of CRC such as stages, chemoresistance, and survival rates. The study was performed on total RNA that was isolated from whole blood of 40 healthy and 63 CRC subjects. The expression of specific lncRNAs (PANDAR, MALAT1, CCAT1, PCAT6, UCA1, MEG3, CCAT2 and BCAR4) was measured by real time PCR. Interestingly, (MALAT1), (CCAT1) and (PANDAR) were significantly up regulated with 1.86, 4.54 and 4.68 fold changes (P<0.05) respectively, in the blood of CRC patients compared to healthy individuals. Other lncRNAs (CCAT2, MEG3, UCA1, BCAR and PCAT6) had also shown increasing expression in blood samples of CRC patients by more than two folds, however, this difference was not significant. In conclusion, the expressions of several lncRNAs were found differently in CRC patients. These results suggest that the expression of (MALAT1), (CCAT1) and (PANDAR) may serve as potential biomarker for CRC diagnosis. |
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Supervisor |
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Dr. Ayat Badr Al-Ghafari |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1439 AH
2017 AD |
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Co-Supervisor |
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Dr. Hani Mohammad Zubair Choudhry |
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Added Date |
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Wednesday, December 13, 2017 |
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Researchers
| حليمه السعدية أمان الله صديقي | Siddiqui, Halima Saadia Aman Allah | Researcher | Master | |
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