Document Details

Document Type : Thesis 
Document Title :
Molecular and Microbial Studies of infant food to Detect Microbial Contamination
دراسات جزيئية وميكروبية لأغذية الاطفال للكشف عن التلوث الميكروبي
 
Subject : Faculty of Sciences 
Document Language : Arabic 
Abstract : Microbial contamination of infant food is a common problem and sometimes life-threatening for new born babies throughout the world. The main aim of our study was to isolate pathogen bacteria present in cereal powder using conventional (Biochemical) and Modern techniques (16s rRNA gene analysis and RAPD analysis). About 32 types of powder (Cereal) was used during our study, which was collected from different Pharmacies of Makkah province, Saudi Arabia. Samples were analyze aerobically and anaerobically in order to isolate the bacterial strains. Among 32 samples only three strain was identified as pathogen. 16s rRNA gene analysis showed that two of these strain belong to genus Bacillus and was identified as Bacillus cereus (KX405589), and Bacillus thuringiensis (KX426043), and the third on is belong to Lysinibacillus fusiformis (KX470807), which may be a threat to the baby health. So our study reveals that Molecular techniques such as 16s rRNA gene is a powerful tool to detect pathogenic bacteria in infant food and could be used by industries in order to identify these pathogenic strains. Some of the cereal powder needs to be very good sterilized. Finally, future research should be continue with all other stage of cereal food products to ensure the very good quality and safety. 
Supervisor : Dr. Mohamed Morsi Ahmed 
Thesis Type : Doctorate Thesis 
Publishing Year : 1438 AH
2017 AD 
Co-Supervisor : Dr. Mohamed Hamed Mutawakil 
Added Date : Tuesday, August 15, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
خالد صالح الشيخAlshaikh, Khalid SalehResearcherDoctorate 

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