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Document Type |
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Thesis |
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Document Title |
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Evaluation Of The Antimicrobial Activities Of The Biocompatible Nanoemulsion System Against Some Wastewater Pathogenic Microbes تقييم النشاط المضاد للميكروبات لنظام المستحلب النانومتري الحيوي على بعض الميكروبات الممرضة في مياه الصرف الصحي |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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Pathogenic bacteria remain a major health problem because they are responsible for causing a large number of diseases .The main drawbacks for conventional antimicrobial agents are the development of multiple drug resistance and adverse side effect. Nanomaterials, which show antimicrobial activity by themselves and elevate the effectiveness and safety of antibiotics administration, are called “nanoantibiotics”. The main objective of this study was to prepare nanoparticles that would work as new therapeutic agents against pathogenic microbes. The nanoemulsions (NEb and NEc), produced by mixing the surfactants, soya phosphatidylcholine, polyoxyethylenglycerol trihydroxystearate 40 and sodium oleate, cholesterol,1-octanol and aqueous buffer Tris-HCl. Antibiotic loaded NEs( NEb+ Ab and NEc+Ab ) were prepared by adding 0.001g of Cefozon in 1ml. The droplet sizes of all of NE formulations ( NEb and NEc) were found in the range of 4 -10.86 nm and ( NEb+ Ab and NEc+Ab ) were found in the range of 50.31- 166.62 nm, their morphology were spherical and normally distributed, as determined by transmission electron microscope. Antimicrobial activities of the NEs ( NEb, NEb+Ab, NEc and NEb+Ab) were examined against some Garm positive and negative bacteria. The highest antimicrobial activitiy was recorded against Micrococcus luteus (+ve) and Serratia marcescens. (-ve). The mechanism of action of NEs was explored against Micrococcus luteus and Serratia marcescens. The MICs for Micrococcus luteus were 77.9µl /10 ml for NEb, 9.6 µl /10ml for NEb+Ab, 152 µl / 10ml for NEc and 19.2 µl /10ml for NEc+Ab. However in case of using Serratia marcescens, MICs were 39.4 µl /10 ml for NEb, 19.2 µl /10 ml for NEb +Ab, 152 µl /10 ml for NEc and 77.6 µl /10 ml for NEc+Ab. The effect of the two NEs on cell wall composition of Micrococcus luteus and Serratia marcescens, .potassium leakage, cellular respiration, and hydrophobicity were also assessed |
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Supervisor |
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Prof. Magda Mohammad Ali |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1438 AH
2017 AD |
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Co-Supervisor |
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Dr. Reda Hasan Amasha |
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Added Date |
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Thursday, June 15, 2017 |
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Researchers
| هناء علوي العطاس | ALattas, Hanaa Alawi | Researcher | Master | |
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