Document Details

Document Type : Thesis 
Document Title :
ISOLATION AND MOLECULAR IDENTIFICATION OF POLY AROMATIC HYDROCARBONS DEGRADING BACTERIA FROM SAUDI ENVIRONMENT
العزل والتعريف الجزيئي للبكتريا ذات المقدرة على هدم الهيدروكربونات العطرية عديدة الحلقات من البيئة السعودية
 
Subject : Faculty of Sciences > biological sciences department 
Document Language : Arabic 
Abstract : Oil spills are an environmental disaster that not only endangers the local flora and fauna but is also an imminent threat to the human health. This necessitates the remedies that can be out in place to overcome this environmental debacle. Our study focused on the biological remedies to degrade crude oil in the environment and then pin point the microbes that play the most effective role in doing so. For this reason we isolated Twenty three crude-oil-degrading bacteria from oil-contaminated sites in Yanbu region. Based on different biochemical tests, a high growth rate on crude oil, hydrocarbon degrading ability and DCPIP assay, four strains were selected from the 23 isolated strains for further study. The isolates were designated as S3, S4, S5, 4b. All of these four isolates had degradation activity of more than 50 %. The nucleotide sequence of the 16S rRNA gene amplified from these strains showed that these isolated strains belonged to genus Pseudomonas and Nitratireductor. The rate of crude-oil degradation was also corroborated using spectrophotometer and GC-MS after two weeks of cultivation in Bushnell Haas medium. Among the four isolates, strains S5 (Pseudomonas sp ) and 4b (Nitratireductor sp) were very effective in degrading crude oil with 95% and 66% of degradation respectively. In this study we successfully managed to isolate and identify the most active strains with the potential to degrade crude-oil present in the environment in the Kingdom of Saudi Arabia. 
Supervisor : Dr. AMR A, SAMRA 
Thesis Type : Master Thesis 
Publishing Year : 1437 AH
2016 AD 
Co-Supervisor : Prof. SALEH M, ALGARNI 
Added Date : Wednesday, March 23, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عبدالواحد محمـد الفايديALFAIDI, ABDUALWAHED MResearcherMaster 

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