Document Details
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Document Type |
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Article In Journal |
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Document Title |
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Ficus sycomorus latex: A thermostable peroxidase Ficus sycomorus latex: A thermostable peroxidase |
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Subject |
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Biochemistry |
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Document Language |
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English |
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Abstract |
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Peroxidase from sycamore fig Ficus sycomorus latex (POLI) was purified by heat treatment, anion exchange chromatography and molecular exclusion chromatography. The purity was determined from high specific activity (9166 units/mg protein), purification fold (28), RZ value 3.1 and a single band in native polyacrylamide gel electrophoresis (PAGE), sodium dodecyl sulfate (SDS)-PAGE and visualized peroxidase activity on the PAGE. POLI had molecular mass of 43 kDa. Substrates commonly used in immunodiagnostic kits as 2,2-azino-bis [3-ethyl-benzothiazoline-(6)-sulfonic acid] (ABTS), 4-chloro-1- naphthol (4C-1N), o-phenylenediamine (OPD) and 3,3',5,5'- tetramethylbenzidine (TMB) were found to be the best substrates for the enzyme. The Km for catalysis of H2O2 was 1.2 mM. The catalytic efficiency (Vmax/Km) for POLI was found to follow the order: ABTS, 4C-1N, OPD, TMB, guaiacol, paminoantipyrine, o-dianisidine and pyrogallol. The enzyme showed a broad pH optimum ranged from pH 5.5 to 7.0. The optimal temperature for the enzyme was 35 to 40°C. POLI showed highest thermal stability. No loss of enzyme activity was recorded up to 60°C, whereas only 20% of enzyme activity was lost at 70 to 90°C. The thermal inactivation profiles of POLI demonstrated that the enzyme had higher thermal resistance. The peroxidase activity was slightly enhanced by low concentration of Ca2+, Ni+2 and Mg2+ and high concentration of Mn2+. Fe+3, Zn+2, Hg+2 caused slightly inhibitory effects. In conclusion, sycamore fig latex will be a new and potential source for a peroxidase enzyme. |
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ISSN |
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1684-5315 |
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Journal Name |
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African Journal of Biotechnology |
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Volume |
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10 |
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Issue Number |
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76 |
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Publishing Year |
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1432 AH
2011 AD |
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Article Type |
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Article |
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Added Date |
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Saturday, February 18, 2012 |
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Researchers
| صالح أحمد محمد | Mohamed, Saleh Ahmed | Researcher | Doctorate | saleh38@hotmail.com |
| Azza M Abdel-Aty | Abdel-Aty, Azza M | Researcher | Doctorate | |
| Mohamed Belal Hamed | Hamed, Mohamed Belal | Researcher | Doctorate | |
| Mohamed O El-Badry | El-Badry, Mohamed O | Researcher | Doctorate | |
| Afaf S Fahmy | Fahmy, Afaf S | Researcher | Doctorate | |
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