Document Details

Document Type : Thesis 
Document Title :
Stem Cells in Developing Mouse Hepatobiliary System: Histo-immune Study
الخلايا الجذعية أثناء تكوين الجهاز الكبدي الصفراوي للفأر: دراسات نسيجية و مناعية
 
Subject : Faculty of Sciences 
Document Language : Arabic 
Abstract : Adult stem cells are found in and around different organs and tissues of the body in a developing embryo and cellular therapy with liver stem cells and their progeny is a promising new approach which will contribute to gene therapy of liver diseases .So, the aim of this study was to investigate the normal development and structure of liver from the embryo to fetus and finally to adult stage with particular reference to especial type of stem cells (oval cells ).Four ages of mice were used : (1) 13 days of gestation ; 16 days of gestation ; 7-days after birth and 60-days after birth. Analysis of section profiles revealed that liver hematopoiesis reached a peak at E13 and gradually declined till the end of intrauterine life. At E 13 liver composed of hepatic cords with hepatoblasts, at E 16 hepatocyte volume and number increased, at 7-days hepatocytes organized into hexagonal plats and at 60-days hepatocytes organized into hepatic lobules. In portal space at E16 only the vein and bile duct appeared but complete portal area was recorded at 7-days and adult one. Kupffer cells first detected at 7-days. During gestation, cells provided with short profiles of rough endoplasmic reticulum, small ill-defined mitochondria and large amount of ribosomes but after birth, cells provided with long parallel cisternae of rough endoplasmic reticulum, developed mitochondria and noticeable amount of glycogen. Using the CD34+ antibody, we sorted large amounts of candidate liver oval cells with high purity phenotypically defined CD+34 and their amount decrease with aging. Our results have led to a reasonably detailed picture drived the presence of oval cells as well as differentiation of the different hepatic cell types. 
Supervisor : Dr. Saleh Abdul-Aziz Al-Kerayem 
Thesis Type : Master Thesis 
Publishing Year : 1432 AH
2011 AD 
Co-Supervisor : Dr. Awatef Mohammed Ali 
Added Date : Friday, June 17, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
مي محمد عشقانAshgan, Mai MohammedResearcherMaster 

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 29849.pdf pdf 

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