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Faculty of Science and Arts - Khulais
Document Details
Document Type
:
Article In Journal
Document Title
:
A comparative study of first-derivative spectrophotometry and column high-performance liquid chromatography applied to the determination of repaglinide in tablets and for dissolution testing
دراسة مقارنة الطيف المشتق الأول والكروماتوغرافيا عالية الأداء لتحديد repaglinide في أقراص الرباغلنايد لاختبار التركيز وانحلال الدواء
Subject
:
Pharmaceutical Analytical Chemistry
Document Language
:
English
Abstract
:
A fast and reliable method for the determination of repaglinide is highly desirable to support formulation screening and quality control. A first-derivative UV spectroscopic method was developed for the determination of repaglinide in tablet dosage form and for dissolution testing. First-derivative UV absorbance was measured at 253 nm. The developed method was validated for linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ) in comparison to the U.S. Pharmacopeia (USP) column high-performance liquid chromatographic (HPLC) method. The first-derivative UV spectrophotometric method showed excellent linearity [correlation coefficient (r) = 0.9999] in the concentration range of 1-35 μg/mL and precision (relative standard deviation <1.5%). The LOD and LOQ were 0.23 and 0.72 μg/mL, respectively, and good recoveries were achieved (98-101.8%). Statistical comparison of results of the first-derivative UV spectrophotometric and the USP HPLC methods using the Mest showed that there was no significant difference between the 2 methods. Additionally, the method was successfully used for the dissolution test of repaglinide and was found to be reliable, simple, fast, and inexpensive.
ISSN
:
10603271
Journal Name
:
Journal of AOAC International
Volume
:
91
Issue Number
:
3
Publishing Year
:
1429 AH
2008 AD
Article Type
:
Article
Added Date
:
Wednesday, December 22, 2010
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
بشار الخالدي ماجد شتيوي ياسر البستنجي
Bustanji, Y, AlKhalidi, B Shtaiwi, M
Researcher
Doctorate
majedshtaiwi@yahoo.co.uk
Files
File Name
Type
Description
28240.pdf
pdf
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