Document Details

Document Type : Thesis 
Document Title :
The Evaluation of HER-2 Amplification by Florescence In Situ Hybridization (FISH) in Saudi Females with Breast Cancer
تقيم تضاعف جين/ HER-2 بواسطة التهجين الموضعي الوميضي لدى النساء السعوديات المصابات بسرطان الثدي
 
Subject : Biochemistry 
Document Language : Arabic 
Abstract : The HER-2 oncogene encodes a transmembrane tyrosine kinase receptor with extensive homology to the epidermal growth factor receptor. Although, HER-2 has been widely studied in breast cancer, some of the aspects related to biochemistry, were lacking or scanty. Therefore, in the present work, the association of HER-2 gene and protein abnormalities on Saudi female with breast cancer has been studied by immunohistochemistry and fluorescence in situ hybridization. The study also focuses on to explore the present outcome in antibody-based therapies directed against the HER-2 protein and assess the potential as a new modality for breast cancer treatment. The current research design employed unselected stage I, II, and III breast cancer patients (N = 32). All samples were put to tested for HER-2 gene amplification by FISH in paraffin-embedded, formalin-fixed archival material using in HER-2 overexpression by IHC. The efficacy of fluorescence in situ hybridization (FISH) incorporated in detecting the HER-2 amplification further helped in comparison with the HER-2 overexpression detected by immunohistochemical assay. The fact that a considerable number of subjects responded positively by either FISH (35.7%) or IHC (21.42%), a discrepancy apparently- can beexplained on the basis of expected loss of IHC sensitivity associated with tissue fixation, embedding. The studies are furthered compared viz-a-viz with cotemporary findings. 
Supervisor : Dr. Sahirah Ahmed Lary 
Thesis Type : Master Thesis 
Publishing Year : 1428 AH
2007 AD 
Co-Supervisor : Dr. Mohammed Hussein Al-Qahtani 
Added Date : Tuesday, June 10, 2008 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
هبة أحمد الخطابيAlktabi, Heba AhmedResearcherMaster 

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